If you’ve ever done a science experiment, you probably know this feeling. You’ve followed the protocol carefully, measured everything twice, and… your results still don’t look anything like they’re supposed to. Maybe your gel didn’t show any bands, your bacteria didn’t grow, or your “blue” solution turned a weird shade of gray.
When things go wrong in the lab, it’s easy to feel frustrated, but here’s the secret every scientist knows: failed experiments are still valuable experiments. Science isn’t about getting the “right” answer. It’s about asking questions, testing ideas, and learning from what happen, even when it’s unexpected.
1. Stay Calm and Observe
First, take a deep breath. Don’t rush to start over right away. Step back and take a good look at what happened in the protocol, and what you wrote down in your lab notebook.
- Did your reaction change color faster (or slower) than expected?
- Was there a sudden change in temperature, pH, or appearance?
- Did every sample behave the same way, or was one different?
- Did your controls work? Did you establish good controls before starting the experiment?
Even mistakes can reveal useful data. Careful observations help you understand how things went wrong, and might even lead you to a new discovery.
2. Check Your Steps
Go back through the procedure one step at a time. Ask yourself:
- Did I measure everything correctly?
- Were my reagents fresh and properly labeled?
- Was my equipment calibrated?
Small details like using the wrong pipette volume or forgetting to mix a sample can cause big changes in results. Don’t beat yourself up! Scientists triple-check everything because everyone makes these mistakes sometimes!

3. Compare with Your Controls
Controls are a necessity when troubleshooting. A positive control shows what should happen when everything works, while a negative control shows what happens when it doesn’t. If your positive control also failed, that tells you something was wrong with your reagents or setup — not your hypothesis. But if your control worked and your experimental sample didn’t, that’s a clue that your variable (the thing you changed) affected the outcome.
4. Ask for Another Perspective
Science is collaborative! Talk with your lab partners, teacher, or classmates. Sometimes, a fresh set of eyes can spot something you missed. Maybe someone else used the same reagents and got different results, or maybe they’ve seen this problem before and can suggest a fix. Learning how to troubleshoot together is one of the best skills you can gain in science.
5. Record Everything
Even if the experiment didn’t go as planned, write down exactly what happened in your lab notebook, i including what went wrong. Often, repeating the same “failed” experiment with just one small change, like a different temperature, a fresh reagent, or an extra rinse step, can completely fix the problem.
6. Reflect and Redesign
Now that you know what happened, think about how you’d change the experiment next time.
- What’s one variable you could adjust?
- Are there extra controls that would help test your theory?
- Do your results suggest a new question?
This is where real science happens, when we turn our setbacks into insights. Every failed experiment moves you closer to understanding how and why something works.
Science is Messy — and That’s the Point
Some of the biggest discoveries in history came from experiments that didn’t go as planned. Penicillin, X-rays, and even microwave ovens were all found by accident. So if your results surprise you, that doesn’t mean you failed — it means you’re doing real science.
The next time something goes wrong, remember: every mistake is data, and every setback is a step toward discovery.


