C. elegans – Trailblazers in Science, and in the Classroom

The model organism Caenorhabditis elegans (C. elegans) are trailblazers. These tiny, transparent roundworms have played an outsized role in our study and understanding of life. Here are five scientific firsts that make these worms legendary: 1. First multicellular organism to…

Common Lab Questions: GFP Transformation and Purification

Figure 1: Ribbon structure of the GFP protein. The chromophore is highlighted. Green Fluorescent Protein (GFP) is a naturally occurring protein that emits a green light when exposed to certain wavelengths of light. It's an important component in many of…

GFP Transformation Resource Roundup

One of the most impactful experiments in the Biotechnology Laboratory is Kit #223, Transformation of E. coli with Green Fluorescent Protein (GFP). We love this experiment for many reasons: green, glowing bacteria, a visual link between genotype and phenotype, and…