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Figure 1: Ribbon structure of the GFP protein. The chromophore is highlighted. Green Fluorescent Protein (GFP) is a naturally occurring protein that emits a green light when exposed to certain wavelengths of light. It's an important component in many of…
Read More Common Lab Questions: GFP Transformation and Purification
One of the most impactful experiments in the Biotechnology Laboratory is Kit #223, Transformation of E. coli with Green Fluorescent Protein (GFP). We love this experiment for many reasons: green, glowing bacteria, a visual link between genotype and phenotype, and…
Every Friday on Twitter, scientists from around the world post their favorite glowing pictures on Twitter using the hashtag #FluorescenceFriday. So, what is fluorescence? And how can you study it in your classroom? First, fluorescence is defined as "the emission…
Ever since its discovery, GFP or green fluorescent protein, has been widely used in cell and molecular biology laboratories due to its versatility and effectiveness. This powerful research tool was first discovered in the jellyfish Aequorea victoria, and expresses its…
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