If you didn’t already know, Edvotek has an extensive library of biotechnology training videos to help with teacher preparation and student learning. Here is the list of the top 10 videos of 2024, from 10 to one.
- EdvoTech Tips: How to Read Product Labels and Safety Data Sheets. Product labels and safety data sheets are an important way that companies like Edvotek can communicate with their customers about the best way to handle and store chemicals in your classroom. In this video, we discuss each section of the SDS and what it means.
- Packing a Chromatography Column. Chromatography is a biotechnology technique that allows us to separate molecules by size, shape, charge, or other physical properties. In this video, you will learn how to pack a column used for chromatography.
- EdvoTech Tips: What is a DNA Ladder and how do you use it? Agarose gel electrophoresis is a biotechnology technique that uses electricity and a porous gel matrix to separate mixtures of DNA molecules into discrete zones, or bands, based on its size. As the length of a DNA molecule increases, the distance it can travel decreases. So we find larger molecules at the top of the gel, and smaller molecules at the bottom. But how do you know the size of your DNA fragments after electrophoresis? You use a DNA ladder, a common reagent used in agarose gel electrophoresis. This reagent allows us to determine the size of our experimental DNA fragments after they are separated by electrophoresis. In this video, we will discuss the DNA ladder — why we use them, how we use them, and how not to use them! We hope this helps you achieve success in your next electrophoresis experiment.
- Separating Proteins using SDS Polyacrylamide Gel Electrophoresis. SDS Polyacrylamide Gel Electrophoresis is a technique that allows us to separate protein molecules by size. In this video tutorial, we show you how to perform electrophoresis of protein samples. The proteins have been denatured using Sodium Dodecyl Sulfate and reducing agents before electrophoresis, which allows us to accurately determine the size of the polypeptide chain.
- Performing the Polymerase Chain Reaction (PCR). The Polymerase Chain Reaction, or PCR, is a technique that allows researchers to quickly create many copies of a specific region of DNA in vitro.
- Performing Agarose Gel Electrophoresis. Electrophoresis is a technique that allows us to separate DNA molecules by size. In this video tutorial, we show you how to perform electrophoresis using brightly colored dyes. Like DNA, the dyes are negatively charged, so they move through the gel towards the positive electrode.
- Isolation of DNA from Human Cheek Cells. Before we can perform many molecular biology and biotechnology experiments, we must isolate DNA from cells. In this video, cheek cells are homogenized in a solution that releases the DNA from the cell by breaking down cellular and nuclear membranes in a process called lysis.
- Preparing an Agarose Gel For Electrophoresis. Electrophoresis is a technique that allows us to separate DNA molecules by size. In this video, we show you how to prepare an agarose gel for electrophoresis.
- Transformation of E. coli with Plasmid DNA. In the laboratory, scientists can force bacteria like E. coli to acquire exogenous DNA from the surrounding environment through a process called transformation. The newly acquired genetic information is both stable and heritable.
- The Enzyme Linked Immunosorbent Assay (ELISA). The ELISA, or Enzyme-Linked ImmunoSorbent Assay, is an analytical biochemistry technique that uses antibodies to detect the presence of specific biomolecules (i.e. peptides, proteins, antigens and hormones) in a complex sample. These samples can be single proteins or complex mixtures like cellular lysates. In this video, we will show you the basics of how to perform an ELISA in your classroom laboratory.
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