GFP Transformation Resource Roundup

One of the most impactful experiments in the Biotechnology Laboratory is Kit #223, Transformation of E. coli with Green Fluorescent Protein (GFP). We love this experiment for many reasons: green, glowing bacteria, a visual link between genotype and phenotype, and…

What is transformation efficiency and why is it important?

Transformation is highly inefficient—only one in every 10,000 cells successfully incorporates the plasmid DNA. However, since a billion cells are used in a transformation experiment, only a few cells must take up the plasmid to achieve a positive outcome. We can use the data from our experiment to calculate the transformation efficiency!