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Transformation is highly inefficient—only one in every 10,000 cells successfully incorporates the plasmid DNA. However, since a billion cells are used in a transformation experiment, only a few cells must take up the plasmid to achieve a positive outcome. We can use the data from our experiment to calculate the transformation efficiency!
We want to thank everyone who participated in our "Spooky Science" Pumpkin Carving Contest! The entries were fun and creative and we can't wait to host a 2022 contest. See below for some of our favorite entries and scroll to the bottom for our winner!
Practice, Practice, Practice! If you are a beginner, practice your sterile technique using water or another liquid, like LB medium. Practicing the motions and seeing how your hands need to move under the hood before starting your culture will prove…
One of the most common model organisms used in laboratories is the nematode: Caenorhabditis elegans. This nematode species is great for studying toxicity responses, modeling human disease, studying aging and much more. They are simple to take care of, have…
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