Biotechnology Basics: SDS Polyacrylamide-Gel Electrophoresis

What is SDS-PAGE? SDS polyacrylamide-gel electrophoresis, or SDS-PAGE, is a technique that is used to separate proteins according to their molecular weight.  What do I need to separate proteins?  In addition to your protein sample, you will need:  Sodium Dodecyl…

Biotech Basics: DNA Ligase – Building a Bridge with DNA

DNA ligase is one of the most important, but least celebrated, enzymes used in modern biotechnology. This enzyme plays a very important role in DNA replication and repair by acting like a “glue” that can connect different DNA fragments to…